50th Annual Naff Symposium: The Mechanobiology of the Genome

mnlo230 — Tue, 22 Apr 2025 19:30:16 +0000

50th Annual Naff Symposium: The Mechanobiology of the Genome mnlo230 Tue, 04/22/2025 - 03:30 pm

Read more about 50th Annual Naff Symposium: The Mechanobiology of the Genome

49th Annual Naff Symposium: Energetic Foundations & Futures of Life

mnlo230 — Fri, 12 Apr 2024 13:14:41 +0000

49th Annual Naff Symposium: Energetic Foundations & Futures of Life

The Department of ��vlog�ٷ�� held the 49_^th Annual Naff Symposium on March 28, 2024. This year’s topic was Energetic Foundations & Futures of Life.

Speakers:

mnlo230 Fri, 04/12/2024 - 09:14 am

Read more about 49th Annual Naff Symposium: Energetic Foundations & Futures of Life

Towards Photonics-Enhanced Molecular Spectroscopy: Label-free Observation of Single Solution-Phase Molecules using Optical Microcavities

mnlo230 — Tue, 12 Dec 2023 17:47:46 +0000

Towards Photonics-Enhanced Molecular Spectroscopy: Label-free Observation of Single Solution-Phase Molecules using Optical Microcavities mnlo230 Tue, 12/12/2023 - 12:47 pm

Abstract: The vast majority of chemistry and biology occurs in solution, and new label-free analytical techniques that can help resolve solution-phase complexity at the single-molecule level can provide new microscopic perspectives of unprecedented detail. Here, we use the increased light-molecule interactions in high-finesse fiber Fabry-Pérot microcavities to detect individual biomolecules as small as 1.2 kDa (10 amino acids) with signal-to-noise ratios >100, even as the molecules are freely diffusing in solution. Our method delivers 2D intensity and temporal profiles, enabling the distinction of sub-populations in mixed samples. Strikingly, we observe a linear relationship between passage time and molecular radius, unlocking the potential to gather crucial information about diffusion and solution-phase conformation. Furthermore, mixtures of biomolecule isomers of the same molecular weight can also be resolved. Detection is based on a novel molecular velocity filtering and dynamic thermal priming mechanism leveraging both photo-thermal bistability and Pound-Drever-Hall cavity locking. This technology holds broad potential for applications in life and chemical sciences and represents a major advancement in label-free in vitro single-molecule techniques.

Date:

Friday, February 23, 2024 - 04:00 pm - Friday, February 23, 2024 - 05:00 pm

Location:

CP 114

Tags/Keywords:

seminar series

chemistry

Event Series:

��vlog�ٷ�� Department Seminar

Read more about Towards Photonics-Enhanced Molecular Spectroscopy: Label-free Observation of Single Solution-Phase Molecules using Optical Microcavities